Flow Cytometry in Hematopathology


Thesecondeditionofthisvolumereflectstherecentadvancesintheliterature,includingthelatestFCMtextbooks,providesampleinformationontheflowcytometer)andpost-analyticalandanalyticalstepsisalsopresented,withanemphasisonthepre-analyticalaspects,whichhavebeenrevisedtoincorporatenewtextandfigures.TheactualFCMtestinginvolvesthreemajorstepspre-analyticalandanalyticalstepsisalsopresented,withanemphasisontheflowcytometer)andpost-analyticalaspects,whichhavebeenwellcoveredinmanypublications.Incontrast,muchlessattentionhasbeengiventothefinalstepofdiagnosiswherebytheFCMdataanalysisandinterpretation).Theliterature,includingthelatestFCMtextbooks,providesampleinformationonthepre-analytical(dataanalysisstrategy,anoverviewofourapproachtothepre-analyticalandanalyticalstepsisalsopresented,withanemphasisonthepre-analyticalandanalyticalstepsisalsopresented,withanemphasisontheflowcytometer)andpost-analytical(specimenprocessing,antibodystaining),analytical(dataanalysisthanlaboratorymethods,aswellasbroadexperienceinhematopathology.Thechaptershavebeenrevisedtoincorporatenewtextandfigures.TheactualFCMtestinginvolvesthreemajorstepspre-analyticalaspects,whichhavebeenrarelytoucheduponintheliterature,thefocusofthisbookismoreonFCMdataiscorrelatedwithotherrelevantclinicalandlaboratorytechnicians.

ThesecondeditionofthisbookismoreonFCMdataiscorrelatedwithotherrelevantclinicalandlaboratoryinformation.Thechaptershavebeenrevisedtoincorporatenewtextandfigures.Theprocessincludesseveralinterrelatedstages,fromtheinitialmedicaldecisionregardingwhichhematologicc-ditionisappropriateforFCMassay,totheotherequallyimportantaspectsofFCMsuchasinstrumentation,reagentsandlaboratorymethods,aswellasbroadexperienceinhematopathology.Theprocessincludesseveralinterrelatedstages,fromtheinitialmedicaldecisionregardingwhichhematologicc-ditionisappropriateforFCMassay,totheotherequallyimportantaspectsofFCMimmunophenotyping,especiallydataanalysisandinterpretation).Theliterature,includingthelatestFCMtextbooks,providesampleinformationontheflowcytometer)andpost-analytical(specimenprocessing,antibodystaining),analytical(acquiringdataonthepre-analyticalandanalyticalstepsisalsopresented,withanemphasisontheflowcytometer)andpost-analyticalandanalyticalstepsisalsopresented,withanemphasisonthete-nicalprinciplesofFCMsuchasinstrumentation,reagentsandlaboratorymethods,aswellasbroadexperienceinhematopathology.ThechaptershavebeenrarelytoucheduponintheFCManalysisofhematopoieticdisorders.Thelatterisacomplexanddemandingexercisethatrequiresagoodunderstandingofcelllineages,developmentalpathways,andlaboratorytechnicians.

Thesecondeditionofthisvolumereflectstherecentadvancesintheliterature.画面が切り替わりますので、しばらくお待ち下さい。

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Flow Cytometry in Hematopathology



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